Scientific Program

Conference Series Ltd invites all the participants across the globe to attend 4th International Conference on Bioprocess and Bio Therapeutics Houston, Texas, USA.

Day 1 :

OMICS International Bioprocess 2016 International Conference Keynote Speaker Gerd Kaupp photo
Biography:

Gerd Kaupp has completed his PhD from Würzburg University and Post-doctoral studies from Iowa State University, Lausanne University, and Freiburg University. He held a Full-Professorship till 2005 in Oldenburg Germany, and privately continues his research on Wasteless Solid-State Chemistry (since 1984), AFM on rough surfaces (since 1988), the non-stochastic but versatile and better resolving sub-diffraction-limit microscopy for unstained non-fluorescing materials of all types (resolution <10 nm, since 1995), and nano-indentations (since 2000). He has published more than 300 papers in renowned journals and has been serving as an Editorial Board Member of several scientific journals.

Abstract:

The present hype with stochastic imaging of fluorescence dyes like STED, PALM, etc., providing only slightly submicroscopic optical resolution, had long been preceded by uncomplicated and much better resolving apertureless shear force SNOM (scanning near-field optical microscopy). Only the latter technique applies to all types of unstained flat or rough real-world materials' surfaces (dielectric, semi-conductive, metallic, fluorescing, non-fluorescing) at local optical resolution down to <9 nm, and it additionally provides topography. It is versatile, easy, and cheap. The artifacts of apertured SNOM are avoided. Basically, an uncoated illuminated sharply tapered dielectric waveguide tip of a shear force AFM is vibrated at shear force distance and the light reflected back to the silica waveguide is coupled out, measured, and/or diffracted. A commercial laser puller provides 10-20 nm end radii at almost no cost. Metal coating is unnecessary due to our unexpected physical effect of strong materials' dependent near-field enhancement of reflectivity (2-50 fold; not only for metals). This provides chemical contrast. We resolve local molecular reactions on crystals, characterize nanoparticles, measure local Raman- or fluorescence-spectra for identifications and diffusion coefficient determinations, distinguish organelles in bio-cells, resolve details within organelles, detect/localize cancer, judge blood bag performance and nano-pitting of implant materials. Nothing of that is available from the expensive stochastic techniques. Furthermore, these chemically bind fluorescing dyes to biomaterial, which inevitably changes their structure (hydrogen bonding, coiling, etc.). Thus, any conclusions from their highly acclaimed stochastic images urgently require reality check by the preceding superior and more versatile apertureless SNOM.

Break:
Group photo and Coffee break 10:55 -11:20@Foyer

Keynote Forum

Sivakumar Ganapathy

Department of Engineering Technology, College of Technology, University of Houston, Houston, TX 77204, USA.

Keynote: Upstream bioprocessing of natural colchicine

Time : 11:20-11:55

OMICS International Bioprocess 2016 International Conference Keynote Speaker Sivakumar Ganapathy photo
Biography:

Biography Sivakumar’s research is primarily focused on biotech implications and applications of high-value natural products. He has extensively studied the plant-based small molecules pathway biochemistry, synthetic biology and metabolic & bioprocess engineering. He is internationally recognized in the field of biopharmaceuticals and a pioneer in industrial-scale production of bioactive molecules. He has over 40 publications. He is also on the editorial board of several journals. He serves as an expert of grant proposals as well as numerous scientific journals. His laboratory focuses on metabolic and bioprocess engineering of colchicine pathway and developing potential anticancer medicine. In addition, his group is interested in developing biofuels to address energy and environmental problems. Additional information can be found at http://tech.uh.edu/ganapathy

Abstract:

Plant-based colchicine profoundly benefits human health. Demand for ultra-pure natural colchicine, however, is unlikely to be met through conventional production. A new bioprocessing platform has been established using specialized biorhizomes with comprehensive specific-enzymes that catalyze the construction of biogenic functionalized intermediates that convert to colchicine. Biorhizome is a type of compressed scale leaf-derived rhizome tissue, culturally persuaded in vitro. These asexually produced rootstocks, whose buds develop new lateral shoots and adventitious roots that serve as reproductive and storage organs as well as biofactories, are used to biosynthesize high-value pharmaceuticals and others.  Additionally, the sprouting biorhizome carries the same genetic code as its parent plant and is totipotent, and thus can be used to study plant metabolic processes. This presentation will focus on discovery of unique biorhizome platform that have evolved toward total biosynthesis of high-value natural colchicine drug.  

Keynote Forum

Frank Emmrich

University of Leipzig, Germany

Keynote: Perspectives and new process developments in cell therapy

Time : 11:55-12:30

OMICS International Bioprocess 2016 International Conference Keynote Speaker Frank Emmrich photo
Biography:

Frank Emmrich is a Professor of Clinical Immunology and Head of the Fraunhofer Institute for Cell Therapy and Immunology (IZI) Leipzig since 2005. He has published more than 270 papers in reputed journals. He is Member of numerous scientific societies, advisory boards and Project Adviser for various research funding organizations. He has served two election periods as Member of the German National Ethics Council. He is a Professor for Clinical Immunology at the University of Leipzig. His special interest is T Cell Immunology and Immunotolerance. He is currently the Chairman of the Regenerative Medicine Initiative Germany (RMIG

Abstract:

About 20 years were needed to implement the invention of monoclonal antibodies with clinical and industrial acceptance for novel therapies, and another 10 years for reaching blockbuster level. Now the time has come to welcome cell therapies at the clinical stage with complex procedures that have been developed throughout the last 10 to 15 years. Dendritic cell vaccines, NK cells and chimeric antigen receptor (CAR) T cells are being explored in various configurations in clinical studies. Connected with these developments are new challenges. Certain regulatory authorities find it difficult to handle processes with mixed categories like cell therapy and gene therapy, or combinations of drugs with medical devices. On the manufacturing site originates a demand of for reduction product costs by drastically reducing manual processing steps and implementing automation. Moreover, a better understanding of mechanisms at the molecular and cellular level may reduce costs and risks of new therapeutic procedures for patients. It is well established that anti-CD4 antibody treatment of helper T cells would induce immunological tolerance when applied in the ongoing phase of an immune response. We studied the mechanism and could further develop the procedure to an applicable technology that allows extracorporeal treatment of T cell containing allotransplants thus reducing treatment risks and product costs significantly. If applied in the field of organ transplantation and transplantation antigens the procedure may be useful for preventing graft rejection of organ transplants or the graft versus host disease (GvHD), the most severe adverse reaction upon allogeneic stem cell transplantation.

  • Video Presentation
Location: Windsor - I

Session Introduction

Ahmed Mubarak Alsobaai

Hadhramout University, Yemen

Title: Production of bio-ethanol from mesquite tree using fermentation process

Time : 12:30-12:50

Speaker
Biography:

Ahmed Mubarak Alsobaai has completed his PhD from University Science University. He has published more than 20 papers in reputed journals and has been serving as a reviewer of repute. 
 

Abstract:

This work investigated the production of bio-ethanol from mesquite tree using fermentation process. Mesquite common term given to all the multiple disparate plant species of the genus (Prosopis) is a legume family member (Leguminosae). From the basic local studies, the area which is covered by this plant is estimated to be about (30,000-24,000) hectare, and one hectare contains about 500 trees, and every tree can produce about 20 kg from the kroon (fruits) annually. The number of trees in Yemen is about (1,250,000-1,500,000) trees in 2014. In the first step, the hemicellulose fraction of the biomass is broken down into simple sugars. A chemical reaction called hydrolysis occurs when dilute sulfuric acid or by liquid hot water is mixed with the biomass feedstock. In this hydrolysis reaction, the complex chains of sugars that make up the hemicellulose are broken, releasing simple sugars. The complex hemicellulose sugars are converted to a mix of soluble five-carbon sugars, xylose and arabinose, soluble six-carbon sugars, mannose and galactose. A small portion of the cellulose is also converted to glucose in this step. In the second step, cellulase enzymes are used to break the chains of sugars that make up the cellulose, releasing glucose at temperature of 45-50ºC. The glucose is converted to ethanol, through a fermentation process. The ethanol recovery percentage is 95%.

Break:
Lunch break 13:00-13:50@Churchill
  • workshop
Location: windsor-I

Session Introduction

Sabah Alwachi

American University of Beirut, Lebanon

Title: Calpain activity and sperm penetration assay in unexplained infertile man

Time : 13:50-14:50

Speaker
Biography:

Sabah Alwachi has completed his PhD from Edinburgh University, UK.  He is the co-director of International Cultural Relations, University of Baghdad, Head of Biology department and a Professor of Physiology. He has published 16 scientific books and more than 51 research papers in reputed journals. He has also supervised 66 PhD and MSc Post-graduate students, has been serving as an Editorial Board Member of many scientific journals and a member of many world scientific societies

Abstract:

Association of sperm calpain, a calcium dependent protease, with cell fusion and signal transduction processes can take place during penetration of the oocyte. This experiment was done to examine the sperm function, sperm penetration assay parameters and to assess the effects of calpain on the fertilization capacity. The study was carried out on infertile men especially the unexplained ones as well as fertile men through assisted reproductive technologies like ICSI and IVF, and using hamster oocytes as a model for human. The results showed a highly significant difference in calpain activity parameters between the 2 groups and not very significant within the same group. It was concluded that high calpain activity is associated with high sperm function and penetration parameters indicating high potential of oocyte fertilization.

  • Biochemical Engineering|Bioprocess and Product Development
Location: windsor-I
Speaker

Chair

Frank Emmrich

University of Leipzig, Germany

Session Introduction

K Alba

University of Houston, USA

Title: Fluid dynamics and scale-up of pharmaceutical molecule

Time : 14:50-15:15

Speaker
Biography:

Dr. Kamran Alba received his Ph.D. in 2013 from Mechanical Engineering Department of The University of British Columbia. His main research area lies in the field of experimental and analytical fluid dynamics with applications vastly found in energy, food-processing, mining and biology fields. The fluid flows studied range from multiphase, suspension and buoyant systems to coating and co-extrusion processes. He is currently an Assistant Professor in Department of Engineering Technology of The University of Houston and the manager of Complex Fluids Laboratory.

Abstract:

Therapeutic molecules can be produced from plants growing in bubble column reactors. The basic mechanism involves injecting a gas (typically air or nitrogen) through a liquid medium surrounding the biomass. It has been found that the amount of drug produced strongly depends on the flow parameters such as tank dimensions, gas inlet flow rate, sparger design, contained solid mass and the volume, density, and viscosity of the surrounding liquid. Current production techniques rely largely on trial and error due to the lack of fundamental knowledge on the multiphase flow in question. We have recently implemented novel non-intrusive flow visualization and velocimetry techniques to understand the underlying mechanisms governing flow mixing. Our experimental methodology consists of laser imaging and particle tracking, revealing detailed information on mixing pattern and vortical structures forming within the flow. In the absence of biomass, bubbly and churn-turbulent flows are found to form at low and high gas injection rates, respectively. In the bubbly regime, two vortices are dominantly formed on the vessel sides which break down into smaller eddy transitioning into the churn-turbulent regime. The results are targeted to be analyzed in dimensionless maps setting eventually a suitable pathway to scale-up the set-up for commercialization and increased production.

Sakae Tsuda

National Institute of Advanced Science and Technology, Japan

Title: Mass preparation of fish antifreeze protein

Time : 15:15-15:40

Speaker
Biography:

Sakae Tsuda has completed his PhD at the Hokkaido University (Japan) and Post-doctoral studies at the University of Alberta (Canada). His research background is Biomolecular NMR, which gave him the skills of Biochemistry, Biophysics and Structural Biology. He is a Chief Senior Researcher of AIST and also a Professor of HU, who published more than 100 papers. His current research target is the antifreeze proteins, which have originally been explored from Japanese organisms in the last 15 years.

Abstract:

At the moment water is frozen, the huge number of single ice crystals are generated in the water to be grown and sticked together to create a multicrystalline state, which is the general ice in our daily use. Antifreeze protein (AFP) has an ability to bind to the single ice crystal, which disturbs the general ice formation, as the AFP-coated ice crystals cannot form the multicrystalline state. This mechanism is thought to solve some technical problems regarding the frozen storage of water-containing materials, such as foods and tissues. The fish-derived AFP can also bind to the lipid bilayer to improve the cell’s viability. This mechanism will also be applied to “cell-pausing”, a short-term (one-two weeks) cell preservation method performed near 0 ºC for use in cell therapies. Hence, we focus on the fish AFP that possesses a wide applicability from industry to medicine, which requires a development of easy method to prepare the massive amount of it. Especially, we need to have the native powder of fish AFP, since it is a mixture of 8-13 AFP molecules (the AFP isoforms) that function together much more effectively than any single isoform. It is theoretically possible to prepare each AFP isoform with the gene technology to make their mixture. However, the yield of the recombinant protein highly depends on each isoform sequence, and the peptide amount in the native mixture is different between the AFP isoforms, implying that we need to know the optimal blending ratio of the isoforms. In the conference, I will introduce our procedure to obtain a large amount of the native fish AFP, and their ability of thermal hysteresis, ice recrystallization inhibition, and the cell preservation under hypothermic (+4°C) temperatures.

Break:
Coffee Break 15:40-16:00@Foyer

Nian Rui

Qingdao Institute of Bioenergy and Bioprocess Technology-CAS, China

Title: Advancing monoclonal antibody production with improved purification technology

Time : 16:00-16:25

Speaker
Biography:

Nian Rui earned a PhD degree in Chemical and Biomolecular Engineering from National University of Singapore in 2008. He is currently a Professor and Leader of Biological Protein Materials Group in Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences (QIBEBT). Prior to joining QIBEBT in 2015, he worked in Downstream Processing Group of Bioprocessing Technology Institute (BTI, A*STAR, Singapore) and focused on the development of next generation bioseparation technology since 2012. He was the Lead Scientist at A-Bio Pharma (Singapore) and took in charge of the downstream process development of various biosimilars in lab-scale and pilot-scale between 2010 and 2012.

Abstract:

Chromatin from dead cells persists in cell culture harvests as heteroaggregates. They bind more strongly to most chromatography adsorbent than most biological products. They reduce binding capacity by blocking pore access and increase peak width by interfering with pore egress. They will also inflate contamination during product elution by leaching of contaminant subsets from still-bound chromatin elements. Chromatin-directed clarification enables many capture options and a reduction of processing steps. In this study, we show that this new clarification method enabled a wide variety of protein A affinity chromatography media to support dynamic capacities that equaled or closely approached values determined with purified IgG. Contaminant content after single protein A step were reduced to <1 ppm HCP, DNA to <1 ppb, protein A leakage to <1 ppm, and aggregates to 1% with an IgG step recovery of 99.4%. Addition of a single polishing step reduced aggregates to <0.1% and all other contaminants beneath their limits of detection, with a step recovery of 96% and overall process recovery, including chromatin extraction, of 90%.